WE’RE MAKING GENE EDITING 5X FASTER WITH MORE EFFICIENT SELECTION, VALIDATION AND EXPANSION.

Current methods for isolating edited clones waste large numbers of cells for just a few insights. Now, Berkeley Lights’ technology and platforms have changed all that.

Gene editing

 

FASTER CLONING AND SELECTION

SINGLE CELL CLONING AFTER GENE EDITING USED TO TAKE MONTHS. OUR PLATFORM MAKES IT HAPPEN IN 5 DAYS.

With the Beacon® platform speeding up Gene Editing, you can make 10X more edits, 5X faster. Rapidly clone up to 16 cell lines in a week. Characterize each clone on our OptoSelect™ chip, and export live cells for expansion, culture, and sequencing.

RAPID CLONING

MONTHS OF LABOR-INTENSIVE CLONING AFTER GENE EDITING ACCELERATES TO JUST 5 DAYS WITH OUR TECHNOLOGY.

 

 

Some cell types are sensitive to hydrodynamic stress or low-density culture conditions. With current methods, you need to maintain large numbers of clones in stacks of well plates to ensure a few survive. In addition, multiple rounds of cloning are frequently required to obtain truly clonal cell lines. But with our technology, it all happens on one OptoSelect chip, in less than a work-week.

CLONING WORKFLOW ON-CHIP

FORGET WELL PLATES. THE NANOPEN™ CHAMBERS ON OUR OPTOSELECT CHIPS EXPAND YOUR EDITED CLONES QUICKLY AND EFFICIENTLY.

Single cell import, clonal expansion, fluorescent labeling, and clonal phenotyping all happen on our chips with a relatively small population of cells.

GENE EDITING IN-DEPTH

CLONING, CULTURE AND PHENOTYPING HAS NEVER BEEN EASIER THAN WITH OUR PLATFORM.

Our NanoPens enable up to 50% of single human primary T cells to expand for further characterization. Putative edited clones are selected with the help of on-chip staining and imaging. Clones are exported to well plates for culture and sequence verification.

DATA OUTPUT

THE BEACON CLONING WORKFLOW QUICKLY PRODUCES VALUABLE DEEP PROFILING DATA AND CLONAL CELL LINES FOR SEQUENCING VALIDATION.

 

 

Off-chip sequencing of editing outcomes in individual clones shows proportions of reads mapping to HDR (magenta), NHEJ (orange), or WT (blue) editing outcomes in each individual clone. Total read count from each clone in the sequencing run is displayed above the allele frequency. Clones with many different genotypes can be identified, including those that integrated the HDR template on both alleles (100% HDR, Clone 1), as well as clones with the same NHEJ edit on both alleles (Clone 8 with a two base-pair deletion).

READ AN ARTICLE ON CELL IDENTIFICATION AND SORTING USING OUR PLATFORM AT THE NCBI SITE.

HOW APPLICATIONS AND TECHNOLOGY COMBINE
ON THE BEACON PLATFORM