FASTER CLONING AND SELECTION
SINGLE CELL CLONING AFTER GENE EDITING USED TO TAKE MONTHS. OUR PLATFORM MAKES IT HAPPEN IN 5 DAYS.
MONTHS OF LABOR-INTENSIVE CLONING AFTER GENE EDITING ACCELERATES TO JUST 5 DAYS WITH OUR TECHNOLOGY.
Some cell types are sensitive to hydrodynamic stress or low-density culture conditions. With current methods, you need to maintain large numbers of clones in stacks of well plates to ensure a few survive. In addition, multiple rounds of cloning are frequently required to obtain truly clonal cell lines. But with our technology, it all happens on one OptoSelect chip, in less than a work-week.
CLONING WORKFLOW ON-CHIP
FORGET WELL PLATES. THE NANOPEN™ CHAMBERS ON OUR OPTOSELECT CHIPS EXPAND YOUR EDITED CLONES QUICKLY AND EFFICIENTLY.
Single cell import, clonal expansion, fluorescent labeling, and clonal phenotyping all happen on our chips with a relatively small population of cells.
GENE EDITING IN-DEPTH
CLONING, CULTURE AND PHENOTYPING HAS NEVER BEEN EASIER THAN WITH OUR PLATFORM.
Our NanoPens enable up to 50% of single human primary T cells to expand for further characterization. Putative edited clones are selected with the help of on-chip staining and imaging. Clones are exported to well plates for culture and sequence verification.
THE BEACON CLONING WORKFLOW QUICKLY PRODUCES VALUABLE DEEP PROFILING DATA AND CLONAL CELL LINES FOR SEQUENCING VALIDATION.
Off-chip sequencing of editing outcomes in individual clones shows proportions of reads mapping to HDR (magenta), NHEJ (orange), or WT (blue) editing outcomes in each individual clone. Total read count from each clone in the sequencing run is displayed above the allele frequency. Clones with many different genotypes can be identified, including those that integrated the HDR template on both alleles (100% HDR, Clone 1), as well as clones with the same NHEJ edit on both alleles (Clone 8 with a two base-pair deletion).