Cell Line Development
Traceable impedance-based single-cell pipetting, from a research set-up to a robust and fast automated robot: DispenCell-S1
SLAS Technology
Hannart, Héloïse, H... Bonzon, David, D
Single-cell isolation is a truly transformative tool for the understanding of biological systems. It allows single-cell molecular analyses and considers the heterogeneity of cell...
High-throughput and automation advances for accelerating single-cell cloning, monoclonality and early phase clone screening steps in mammalian cell line development for biologics production
Biotechnology Progress
Tejwani, Vijay, V... Sharfstein, Susan T
Mammalian cell line development is a multistep process wherein timelines for developing clonal cells to be used as manufacturing cell lines for biologics production...
Microfluidic chip-based single-cell cloning to accelerate biologic production timelines
Biotechnology Progress
Diep, Jonathan, J... Stevens, Jennitte, J
Cell line development (CLD) represents a critical, yet time-consuming, step in the biomanufacturing process as significant resources are devoted to the scale-up and screening...
Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring
Biotechnology and Bioengineering
Jorgolli, Marsela ... Miranda, Less P.
The new and rapid advancement in the complexity of biologics drug discovery has been driven by a deeper understanding of biological systems combined with...
Preclinical development of a molecular clamp-stabilised subunit vaccine for severe acute respiratory syndrome coronavirus 2
Wiley Online Library
Watterson, Daniel... Chappel, Keith J
Efforts to develop and deploy effective vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continue at pace. Here, we describe rational antigen design...
Recent advances in CHO cell line development for recombinant protein production
Science Direct
Borbála Tihany, LászlóNyitra
Recombinant proteins used in biomedical research, diagnostics and different therapies are mostly produced in Chinese hamster ovary cells in the pharmaceutical industry. These biotherapeutics,...
Methods for Estimating the Probability of Clonality in Cell Line Development
Biotechnology Journal
Chen, Chun, C... Goudar, Chetan T, CT
Mammalian cell banks for biopharmaceutical production are usually derived from a single progenitor cell. Different methods to estimate the probability that the cell banks...
Achieving Unique Synergies in Antibody Expression
Genetic Engineering & Biotechnology News
Kravitz, R... Bleck, G;
n/a
Enabling drug discovery and development through single-cell imaging
Expert Opinion on Drug Discovery
Pomerantz, AK... Krucker, T
Single-cell imaging-based assays are an area of active and growing investment in drug discovery and development. This approach offers researchers the capability to interrogate...
Assuring Clonality on the Beacon Digital Cell Line Development Platform
Biotechnology Journal
Le, Kim, K... Stevens, Jennitte, J
During biomanufacturing cell lines development, the generation and screening for single-cell derived subclones using methods that enable assurance of clonal derivation can be resource-...
A novel mammalian cell line development platform utilizing nanofluidics and optoelectro positioning technology
Biotechnology Progress
Le, Kim... Munro Trent
Generating a highly productive cell line is resource intensive and typically involves long timelines because of the need to screen large numbers of candidates...
Using nanoscale bioreactors to characterize sub-populations of CHO clones and screen transfected pools
ECI Digital Archives
Nevill, Tanner... Elms, Phillip
Traditional means to quantify growth and production rates for antibody-expressing CHO lines involve sampling aliquots and supernatants from well plates that have been seeded...
Interrogating cell culture populations for the selection of production cell lines using microfluidic culturing, single cell analysis, and predictive modelling
ECI Digital Archives
Le, Kim... Yaghmour, Sam
Cell line development for manufacturing is a lengthy, multi-step, resource intensive, critical path activity. Attempts to perform in silico modelling and prediction of cell...
Enabling next-generation cell line development using continuous perfusion and nanofluidic technologies (Abstract Only)
ECI Digital Archives
Goudar, Chetan... Munro, Trent
The manufacturing process for a biologic begins with establishing a clonally derived, stable production cell line. Generating a highly productive cell line is time...