T Cell Profiling
Response under pressure: deploying emerging technologies to understand B-cell-mediated immunity in COVID-19
Nature Methods
Woodruff, Matthew C... Sanz, Ignacio
Critical technological advances have enabled the rapid investigations into the immune responses elicited by SARS-CoV-2, the pathogen responsible for the COVID-19 pandemic. We discuss...
TCR validation toward gene therapy for cancer
Methods in Enzymology
Green, Edward W... Platten, Michael
The speed of T cell receptor (TCR) discovery has been revolutionized by barcode-based TCR sequencing approaches that allow the reconstitution of a T cell's...
High-throughput discovery of cancer-targeting TCRs
Methods in Enzymology
Bunse, Lukas... Platten, Michael
The high-throughput discovery of T cell receptors (TCR) enables us to follow the longitudinal course of tumor disease, to monitor immunotherapy-related repertoire alterations, and...
Light-activated cell identification and sorting (LACIS) for selection of edited clones on a nanofluidic device
Communications Biology
Mocciaro, Annamaria... Lavieu, Gregory
Despite improvements in the CRISPR molecular toolbox, identifying and purifying properly edited clones remains slow, laborious, and low-yield. Here, we establish a method to...