Optoelectronic tweezers: a versatile toolbox for nano-/micro-manipulation
Chemical Society Reviews
Shuailong Zhang, Bingrui Xu, Mohamed Elsayed, Fan Nan, Wenfeng Liang, Justin K. Valley, Lianqing Liu, Qiang Huang, Ming C. Wuk and Aaron R. Wheeler
The rapid development of micromanipulation technologies has opened exciting new opportunities for the actuation, selection and assembly of a variety of non-biological and biological...
Shortening the Biologics Clinical Timeline with a Novel Method for Generating Stable, High Producing Cell Pools and Clones
Chad A Hall, Rachel H Kravitz, Karl F Johnson, Nicholas A Sanek, Payel Maiti, Keith R Ziemba, Jia Liu, Dmitri O Andreev, Victoria L Chrostowski, Ian J Collins, Gregory T Bleck
Reducing drug development timelines is an industry-wide goal to bring medicines to patients in need more quickly. This was exemplified in the COVID-19 pandemic...
Broadly neutralizing antibodies target the coronavirus fusion peptide
Dacon, Cherrelle... Tan, Joshua
The potential for future coronavirus outbreaks highlights the need to develop strategies and tools to broadly target this group of pathogens. Here, using an...
Response under pressure: deploying emerging technologies to understand B-cell-mediated immunity in COVID-19
Woodruff, Matthew C... Sanz, Ignacio
Critical technological advances have enabled the rapid investigations into the immune responses elicited by SARS-CoV-2, the pathogen responsible for the COVID-19 pandemic. We discuss...
Traceable impedance-based single-cell pipetting, from a research set-up to a robust and fast automated robot: DispenCell-S1
Hannart, Héloïse, H... Bonzon, David, D
Single-cell isolation is a truly transformative tool for the understanding of biological systems. It allows single-cell molecular analyses and considers the heterogeneity of cell...
A large-scale systematic survey reveals recurring molecular features of public antibody responses to SARS-CoV-2
Wang, Yiquan, Y... Wu, Nicholas C
Global research to combat the COVID-19 pandemic has led to the isolation and characterization of thousands of human antibodies to the SARS-CoV-2 spike protein,...
Single-cell analysis of circulating tumour cells: enabling technologies and clinical applications
Trends in Biotechnology
Radfar, Payar... Warkiani, Majid Ebrahimi
Multimodal analysis of circulating tumour cells (CTCs) has the potential to provide remarkable insight for cancer development and metastasis. CTCs and CTC clusters investigation...
Single-Cell Technologies for the Study of Antibody-Secreting Cells
Frontiers in Immunology
Broketa, Matteo... Bruhns, Pierre
Antibody-secreting cells (ASC), plasmablasts and plasma cells, are terminally differentiated B cells responsible for large-scale production and secretion of antibodies. ASC are derived from...
Single B cell technologies for monoclonal antibody discovery
Trends in Immunology
Pedrioli, A;Oxenius, A
Monoclonal antibodies (mAbs) are often selected from antigen-specific single B cells derived from different hosts, which are notably short-lived in ex vivo culture conditions...
A review: Dielectrophoresis for characterizing and separating similar cell subpopulations based on bioelectric property changes due to disease progression and therapy assessment
Duncan, Josie L... Davalos, Rafael V
This paper reviews the use of dielectrophoresis for high-fidelity separations and characterizations of subpopulations to highlight the recent advances in the electrokinetic field as...
High-throughput and automation advances for accelerating single-cell cloning, monoclonality and early phase clone screening steps in mammalian cell line development for biologics production
Tejwani, Vijay, V... Sharfstein, Susan T
Mammalian cell line development is a multistep process wherein timelines for developing clonal cells to be used as manufacturing cell lines for biologics production...
Microfluidic chip-based single-cell cloning to accelerate biologic production timelines
Diep, Jonathan, J... Stevens, Jennitte, J
Cell line development (CLD) represents a critical, yet time-consuming, step in the biomanufacturing process as significant resources are devoted to the scale-up and screening...
Single-cell electrical characterization
Biosensors for Single-Cell Analysis
Cottet, Jonathan... Caselli, Federica
This chapter provides an introduction to single-cell electrical characterization techniques and related devices, which play a key role in the framework of label-free biosensing...
Bispecific antibodies targeting distinct regions of the spike protein potently neutralize SARS-CoV-2 variants of concern
Science Translational Medicine
Cho, Hyeseon... Tan, Joshua
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern threatens the efficacy of existing vaccines and therapeutic antibodies and underscores...
Miniaturized single-cell technologies for monoclonal antibody discovery
Lab on a Chip
Van Lent... Lammertyn, J
Antibodies (Abs) are among the most important class of biologicals, showcasing a high therapeutic and diagnostic value. In the global therapeutic Ab market, fully-human...
Massively parallel quantification of phenotypic heterogeneity in single-cell drug responses
Yellen, Benjamin B... Hammerbacher, Jeff
Single-cell analysis tools have made substantial advances in characterizing genomic heterogeneity; however, tools for measuring phenotypic heterogeneity have lagged due to the increased difficulty...
Foundational Tools for Building a New Bioeconomy
Journal of Commercial Biotechnology
Levine, Daniel S
While biotechnology has been transforming the diagnosis and treatment of disease, it has also been altering everything from the production of crops to how...
Novel Monoclonal Antibodies and Recombined Antibodies Against Variant SARS-CoV-2
Frontiers in Immunology
Xie, Jiajia... Gao, Yong
The mutants resulted from the ongoing SARS-CoV-2 epidemic have showed resistance to antibody neutralization and vaccine-induced immune response. The present study isolated and identified...