All Publications
Using nanoscale bioreactors to characterize sub-populations of CHO clones and screen transfected pools
ECI Digital Archives
Nevill, Tanner... Elms, Phillip
Traditional means to quantify growth and production rates for antibody-expressing CHO lines involve sampling aliquots and supernatants from well plates that have been seeded...
Interrogating cell culture populations for the selection of production cell lines using microfluidic culturing, single cell analysis, and predictive modelling
ECI Digital Archives
Le, Kim... Yaghmour, Sam
Cell line development for manufacturing is a lengthy, multi-step, resource intensive, critical path activity. Attempts to perform in silico modelling and prediction of cell...
Light-activated cell identification and sorting (LACIS) for selection of edited clones on a nanofluidic device
Communications Biology
Mocciaro, Annamaria... Lavieu, Gregory
Despite improvements in the CRISPR molecular toolbox, identifying and purifying properly edited clones remains slow, laborious, and low-yield. Here, we establish a method to...
Multiparameter Single Cell Characterization of Ovarian Intratumor Heterogeneity
Obstetrics & Gynecology
Beaumont, K;Copperman... E;Sebra, R
INTRODUCTION: Rapid advances in single-cell and deep sequencing technologies has revealed the genetic and functional complexity of bulk tumor tissue, particularly with regard to...
Acoustofluidic Innovations for Cellular Processing On-Chip
Thesis
Ohiri, KA
The advent of increasingly proficient cell handling tools has led to a drastic maturation of our understanding of life on the microscale. Thus far,...
Enabling next-generation cell line development using continuous perfusion and nanofluidic technologies (Abstract Only)
ECI Digital Archives
Goudar, Chetan... Munro, Trent
The manufacturing process for a biologic begins with establishing a clonally derived, stable production cell line. Generating a highly productive cell line is time...
Microfluidic Cell Sorting and Separation Technology
Microsystems and Nanosystems
Lee, Wonhee... Di Carlo, Dino
Cell sorting and separation is widely used as a critical first step for research and clinical applications where it is needed to isolate individual...