OVERVIEW

The Lightning Optofluidic System

Lets you directly visualize the phenotype and function of more than 1000 individual T cells in just days.

News

The Lightning™ System has been named a winner in the Scientist’s Top 10 Innovations for 2019

See Award

The Lightning System Workflows

Lightning enables game changing process improvements and is most popular for the following workflows. Pick your workflow and see the difference:

VISUALIZE

Diverse Cell Interactions

The Lightning system lets you precisely build hundreds of individual cell-cell interactions to perform highly controlled experiments that isolate and investigate individual cell function. This can be applied across many types of experiments including CAR-T construct screening and validation, antigen or TCR discovery and validation, as well as investigation of helper or regulatory T cell function.

Possible interactions:

  • CAR-T cell + Target cell expressing antigen
  • Pan T cell + T2 antigen presenting cell
  • CD8+ T cells + Dendritic cell presenting antigen
  • CD4+ T cells + B cell presenting antigen
  • CD8+ T cells + Treg cell

 

 

MEASURE

Individual Cell Cytotoxicity

Directly measuring T cell cytotoxicity is challenging and generally accomplished by correlating indirect ELISA measurements and target cell proliferation rates. The Lightning enables you to directly measure the activation phenotype (CD137) and the function (IFNɣ secretion) of individual T cells of interest.

This lets you:

  • Rapidly catalog individual T cell variability
  • Simplify cytotoxicity measurements
  • Perform direct tumor killing
  • Run multiplex cytokine assays
RECOVER

Cells of Interest, Alive

You’ve spent time, energy, and resources profiling your T cell population. With the Lightning system, you can recover the most interesting cells for further characterization. Visualize and evaluate your cells and data in our Assay Analyzer software and recover the best cells that meet your export criteria. You can individually unload any single NanoPen™ chamber on the chip or group single cells with similar phenotypes and function for export into a single well of a well plate for downstream analysis.

LIGHTNING technology:

At the core of the Lightning system is a combination of optics and nanofluidics called optofluidics.

The OptoSelect™ chips replace typical well plates. Each OptoSelect chip consists of NanoPen chambers which are like wells on a microplate. This is where cells are deposited and where the assays take place.

Lightning Workflow Modules

The Lightning optofluidic system lets you directly visualize the phenotype and function of 100s to 1000s of individual T cells in just days using the four workflow modules. Light and semiconductor technology combine to move single cells or beads in large numbers and automated microfluidics enable reagent exchange so you can:

Load the Beads

 

Load Your T Cells

 

Load Tumor Cells

 

Watch How They Interact

 

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