B-Cell Antibody Discovery Workflow

Access broad B cell diversity to select better antibody lead candidates in just days.

 

  • In less than 24 hours, using the Beacon single-cell system, GenScript ProBio completed immunized mice processing, immune cell isolation and enrichment, single cell sorting, on-chip binding and blocking screening, which usually takes three months using the traditional hybridoma antibody screening.
    Dr. Brian Min, CEO of GeneScript ProBio
GREATER B CELL DIVERSITY

With the Opto™ Plasma B Discovery Workflow, everything you need to connect antibody function to gene sequence at the single-cell level is available.

Increase your chances of finding rare functional antibody therapeutic candidates by screening the plasma B cell repertoire using the Berkeley Lights Platform.

Rapid Screening of the B Cell Repertoire

Plasma B discovery using the Beacon® optofluidic system bypasses time-consuming and inefficient hybridoma fusion.

 

 

Plasma B cells are screened using binding and functional assays to select only the most qualified antibody lead candidates. OptoSeq™BCR enables automated recovery of broad antibody sequence diversity in under a week.

Down-select Lead Candidates Earlier.

The Opto Plasma B Discovery Workflow enables down-selection of lead candidates through multiple assays for antigen specificity and function.

With a NanoPen™chamber volume of only 250 pico liters, reactions are fast and precise. Complete assays in less than 1 hour, then reset your chips to enable the next assay to begin. Better characterization upfront reduces the expense of having to sequence or clone irrelevant non-functional hits.

ASSAYS THAT RUN IN OUR NANOPEN CHAMBERS

Functional Assay

Ligand Blocking Assay

Cross-Species Assay

Antigen Specific Bead Assay

Link Sequence With Function in Just Two Days

Every antibody sequence can be mapped directly to a known antibody function. Sequence relationships identify individual mutations that confer antigen specificity, cross-reactivity, and function.

 

Discover Thousands of Hits in Days, Not Months.

Beacon System’s OptoSelect™ Chip
Step 1: Cloning
Step 2: Assay
Step 3: CDNA Synthesis
Step 4: Sequence Recovery

Plasma B discovery on the Beacon system enables automated, direct screening of B cells immediately after organ harvest and cell purification.

B cells can be screened from multiple organs (spleen, bone marrow, lymph nodes) and cultured for multiple days in specialized media to enable multiple screens from a single cell sample.

Get in Touch with Berkeley Lights

Let us know what you are looking for and one of our experts will contact you promptly to follow up on your request.