Workflow
Antibody Discovery
Lead molecules against any target at record pace
customer webinars
Workflow in Action
See how our customers apply our antibody discovery workflow to solve real world problems.
Featured:
Abveris on “Lessons Learned from 2+ Years of Discovering Functional Antibodies on the Beacon”
Watch More:
Vanderbilt University on finding SARS-CoV-2 antibodies
DATASHEET
The Opto® Memory B Discovery Rabbit Workflow
Opto® Memory B Discovery on the Beacon® Optofluidic System enables rapid selection of lead molecules by function-forward screening of B cells from immunized animals in under 1 week. While Opto® Plasma B Discovery workflows enable screening of mouse plasma B cells, the Opto Memory B Discovery workflow enables rapid screening of rabbit memory B cells.
Rabbits have unique gene diversification strategies that produce antibodies with high affinity and specificity, making rabbit antibodies uniquely valuable reagents for basic research, diagnostics, and therapeutics development.1 Rabbit polyclonal antibodies are widely used for research applications; however, monoclonal antibodies are often preferred for diagnostic and therapeutic applications due to their well-defined specificity and stability.
Single B cell approaches are an attractive approach for discovery of rabbit monoclonal antibodies as compared with hybridoma and phage display owing to the low fusion efficiency and loss of cognate heavy/light chain pairing in the latter two approaches, respectively. A central challenge to single B cell screening in rabbits is the lack of robust B cell markers that enable purification of rabbit plasma B cells. As a result, rabbit B cell screening approaches are typically performed using memory B cells that must be activated to secrete antibodies for screening purposes.
PRESENTATION
Accessing Broad B Cell Diversity to Rapidly Identify High-Value Rabbit Monoclonal Antibodies
As presented at Antibody Engineering & Therapeutics 2022, by Anupam Singhal, Ph.D., this presentation introduces Berkeley Lights’ newest antibody discovery workflow, the Opto® Memory B Discovery workflow, and shares how customers are using the workflow to screen rabbit memory B cells to rapidly discover rabbit monoclonal antibodies.
Target to lead molecule in weeks, not months
The Opto® Plasma B Discovery workflow on the automated Beacon® system enables high-resolution screening of plasma B cells with unprecedented speed.
- Directly clone B cells from multiple compartments
- Generate diverse hit panels
- Downselect lead candidates with functional characterization
- Recover re-expressed lead molecules in just 1 week
- Accelerate the time to clinic to realize millions in enabled revenue
Rapidly Downselect Lead Molecules and Prepare Libraries for NGS Sequencing
Guaranteed campaign success
Find antibodies against any target by putting function first and accessing broad B cell diversity.
MEMBRANE TARGETS
Direct functional profiling of B cells enables discovery of 10-fold more antibodies against GPCRs and other high-value targets.
© Genovac Antibody Discovery LLC. All rights reserved.
Rare Molecules
Access to different species and B cell compartments to screen greater biodiversity.
High Potency Antibodies
Screening throughput of up to 100,000 B cells from a single sample allows recovery of higher affinity antibodies.
CASE STUDY: FROM DONOR TO LEAD ANTIBODY IN 18 DAYS
Get to the clinic faster
The Opto Plasma B Discovery workflow enables cloning and direct functional profiling of B cells in a single day.
Vanderbilt University delivered sequences of confirmed SARS-CoV-2 neutralizing antibodies to downstream manufacturing partners in just 18 days using this workflow. Two of these antibodies form the basis of an AstraZeneca antibody cocktail for the treatment of COVID-19 currently in Phase III clinical trials.
These significant time savings not only enable a rapid response to emerging pandemics but can also deliver millions of dollars in enabled revenue and return on investment.
Leave no hit behind
Have high hit rates? No problem! Recover every one with OptoSeq Barcoded BCR.
- Screen up to 50,000 plasma B cells and recover 1000s of hits from a single automated 4-chip workflow
- Run multiple workflows to screen up to 100,000 cells from a single sample to recover >10,000 hits
- Recover antibody sequences with high-efficiency using OptoSeq Barcoded BCR
Link sequence to functional profiles
OptoSeq™ Barcoded BCR enables linkage of sequence to function by dual optical and genetic barcoding. Fluorescence signatures of beads are decoded on the Beacon system prior to exporting into specific locations in well plates. The genetic barcodes read by NGS sequencing can then be mapped to each bead, linking every antibody sequence to its corresponding function.
Recover lead molecules, not just sequences
Re-express >1,000 antibodies in 1 week without gene synthesis and cloning.
The Opto BCR Rapid Re-expression kit converts antibody sequences into molecules in just 1 week for plate-based confirmation assays. Amplified cDNA is cloned into expression constructs in one step, enabling expression of >1,000 antibodies for rapid functional confirmation.
Related Resources
View All Antibody Discovery ResourcesDATASHEET: Opto® Memory B Discovery Rabbit Workflow
- Rapid workflow to screen rabbit memory B cells to discover high-value monoclonal antibodies
- Robust activation of rabbit memory B cells using proprietary feeder-free activation media
- Efficient recovery of antigen-specific antibody sequences for downstream characterization and development
DATASHEET: Opto® Plasma B Discovery 4.0 Workflow
- Recovery of 1,000s of hits from over 40,000 individual plasma B cells in a single 4-chip workflow
- Down-selection of lead candidates by functional profiling
- Sequencing and re-expression of >1,000 functionally-characterized antibodies
PRESENTATION: Accessing Broad B Cell Diversity to Rapidly Identify High-value Rabbit Monoclonal Antibodies
Opto® B Discovery on the Beacon® optofluidic system enables rapid selection of lead candidates by function-forward screening of B cells in under 1 week. This presentation will introduce Berkeley Lights’ newest antibody discovery workflow, the Opto® Memory B Discovery workflow, and share how customers are using the workflow to screen rabbit memory B cells to rapidly discover rabbit monoclonal antibodies (as presented at Antibody Engineering & Therapeutics 2023).
Brochure: Lead Molecules Against Any Target. At Record Pace.
Find out how Opto™ Plasma B Discovery on the Beacon optofluidic system allows CROs to scale their business and rapidly deliver lead molecules even against difficult targets.
Brochure: Get to the Clinic First with Best-In-Class Antibody Therapeutics
Find out how Opto™ Plasma B Discovery on the Beacon optofluidic system allows Pharma to succeed against difficult targets and deliver best-in-class antibody therapeutics.
Webinar: A Beacon for Rapid Antibody Discovery against Difficult Targets
Panelists from Genovac, will present case studies of how they have leveraged their core genetic immunization technology and the Beacon optofluidic system to rapidly discover antibodies against challenging targets, such as GPCRs, from a variety of different animal species.
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