Workflow
Cell Line Development
Best cell lines for complex antibody therapeutics with unrivaled clonality assurance in just days.
Best production cell lines
with >99% monoclonality assurance in just days.
Rapid Workflow
Replace 8-12 weeks of challenging well plate steps with Opto™ CLD on the Beacon® optofluidic system. In Cell Line Development (CLD), you are looking for the rare, high-producing clones. For the greatest genetic diversity, you need to screen thousands of cells. The Opto CLD workflow screens thousands of clones with ease in less than a week.
Complex Antibody Molecules
Select top clones for even non-traditional antibody molecules.
The Opto CLD Workflow selects top clones by measuring growth and titers over multiple days of on-chip culture. SpotLight™ assays enable quantification of both traditional and non-traditional antibodies, such as Fc-engineered mAbs, bispecifics, and multispecifics.
>99% monoclonality assurance
Get >99% monoclonality assurance with a single, automated round of cloning.
The Berkeley Lights Opto CLD workflow enables in-line controls to measure clonality during clone recovery. After recovery of each clone, media is flushed into “blank” wells to detect any residual cells. Expand >90% of selected clones from the Beacon system when recovered into 96-well plates, with >99% monoclonality assurance – which is equivalent to four rounds of limiting dilution!
Visual confirmation of monoclonality
Compile a superior data package with simple, visual confirmation of monoclonality
Unlike traditional well-plate approaches, the Opto CLD Workflow provides a visual record of all clones – in an automated fashion – and allows confident assessment of monoclonality. Cells can be visually tracked from the moment they are loaded into NanoPen™ chambers through culture, antibody secretion assays, and eventual recovery of top secreting clones. Imaging provides information about cell size, morphology, and growth rates for all clones over multiple days of on-chip culture.
Export the best from 1000s of clones
The Opto CLD Workflow enables selection of top clones by measuring growth over multiple days of on-chip culture and secretion titers using quantitative assays for both traditional and non-traditional antibodies.
- Measure antibody production from 1000s of clones
- Quantify secretion titers and specific (per-cell) productivity using the Spotlight™ Human Fc and SpotLight™ Human Kappa reagents
- Enrich and recover top-secreting clones for scale-up
Spotlight assay: Simultaneous, quantitative antibody titer measurements on thousands of clones (LEFT). Clones selected based on titer measurements on the Beacon system had 1.5–3-fold higher titers than clones selected using traditional clone picking technology when scaled up to shake flask fed-batch cultures (RIGHT). ©2019 Catalent, Inc. All rights reserved.
Related Resources
View All Cell Line Development ResourcesBrochure: Cell Line Development
Find out how the Opto™ CLD workflow lets you generate the best production cell lines for complex antibody therapeutics with >99% monoclonality assurance in just days.
Cell Line Development Webinar: Digital Week 2019
Learn about improved and expedited cell line development on the Beacon platform from Biogen’s Rachel Ferguson.
Poster: Automated Cell Line Development with Greater than 99% Monoclonality on the Beacon System
This poster explains how automated Cell Line Development with greater than 99% monoclonality can be achieved on the Beacon system.
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