Workflow
Cell Therapy Development
Deliver the right cells to the patient on time.
THE STORY OF YOUR T CELL
Your T cell could be the key to the next powerful therapeutic.
To realize its potential, you need to deeply characterize this cell. The Cell Therapy Development Workflow lets you directly link a single cell’s phenotype and function to its genotype. It only takes one cell to create a powerful therapy. We’ll help you find it.
The Opto™ Cell Therapy Development 1.0 Workflow
Accelerates the creation of better therapeutics by enabling multiple functional assays to be performed simultaneously on 1000s of individual T cells in a matter of days. Deeply characterize, profile, and map your T cell populations at the single-cell level to directly link behavior to gene expression.
Characterize only the cells you want
Berkeley Lights’ workflows allow you to screen desired phenotypes within heterogeneous T cell samples.
Cells can be selectively cloned into NanoPen™ chambers based on size, viability, and other cell surface markers.
Run multiple assays on each cell
Secretion, Killing, and More
The Opto Cell Therapy Development 1.0 workflow allows you to investigate complex cell-cell interactions. Our ever-expanding portfolio of capabilities includes assays to study secretion of multiple cytokines and track killing kinetics, as well as kits that enable TCR sequencing.
Download Datasheets
Cytokine Datasheet (PDF)
Cytotox Datasheet (PDF)
TCRseq Datasheet (PDF)
MEASURE MULTIPLE CYTOKINES
Our Multiplex Cytokine Assays allow simultaneous functional interrogation of T cells as they interact with target cells, giving you insight into the heterogenous mechanisms that regulate cytokine secretion.
Cytokine assays and cell surface markers can be customized.
EXPAND AND VALIDATE FUNCTION OF RARE, ANTIGEN-SPECIFIC T CELLS
The Opto™ Antigen Presenting Bead Kit allows you to expand rare T cells 10x more effectively than traditional methods that use dendritic cells.
Load beads with your target peptide to expand T cells present at frequencies as low as 0.001%. Then, validate and characterize function with our multiple assays.
VISUALIZE TUMOR CELL KILLING
Our Cytotoxicity Assay leverages caspase-3 activation in target cells to visualize the disparate killing behaviors in your T cell samples.
Now, you can develop therapeutics to mediate more effective tumor destruction by the few T cells that matter.
Recover cells of interest alive
After functional characterization, cells of interest can be exported alive for expansion or for further study to link their behavior to genotype.
Connect function to gene expression
Recover cells of interest alive and link unique behaviors to genotype.
The TCRseq Well Plate Kit allows you to recover your selected cells from fewer than 10,000 cells, amplify TCR alpha and beta chains, and sequence only the T cells that are potential therapeutics.
Related Resources
View All Cell Therapy Development ResourcesBrochure: Cell Therapy Development
From expanding rare, antigen-specific T cells to fully characterizing them, learn how our capabilities are making cell therapy development more efficient.
Application Note: Directly Link T Cell Phenotype and Function to Genotype with the Opto Cell Therapy Development 1.0 Workflow
Review use cases for CAR-T cell screening and TCR discovery.
Get in Touch with Berkeley Lights
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