Workflow
Cell Therapy Development
Deliver the right cells to the patient on time.
Maximize Functional Characterization
of Your Precious T Cell Samples
The Opto™ Cell Therapy Development workflow accelerates the creation of better therapeutics by performing multiple functional assays across 1,000s of individual T cells in a matter of days. Deeply characterize, profile, and map your T cell populations at the single-cell level to directly link phenotype to gene expression.
The importance of a single t cell
One T cell could be the key to the next powerful therapeutic.
To realize its potential, you need to deeply characterize it. The Opto Cell Therapy Development workflow lets you directly link single-cell phenotype and function to gene expression. It only takes one cell to create a powerful therapy. We’ll help you find it.
Characterize only the cells you want
Berkeley Lights’ workflows allow you to screen desired phenotypes within heterogeneous T cell samples.
Cells can be selectively cloned into NanoPen™ chambers based on size, viability, and other cell surface markers.
Run multiple functional assays
Characterize polyfunctionality, killing, genotype, and gene expression.
The Opto Cell Therapy Development workflow allows you to investigate complex cell-cell interactions. Our ever-expanding portfolio of capabilities includes assays to study polyfunctionality, cytotoxicity, and to directly link phenotype to genotype and gene expression.
Download Datasheets
Multiplex Cytokine Assay Datasheet (PDF)
Cytotoxicity Assay Datasheet (PDF)
TCRseq Kit Datasheet (PDF)
PROLIFERATE RARE, ANTIGEN-SPECIFIC T CELLS
The Opto™ Antigen Presenting Bead Kit allows you to expand rare T cells 10x more effectively than traditional methods that use dendritic cells.
Load beads with your target peptide to expand T cells present at frequencies as low as 0.001%. Then, validate and characterize function with our multiple assays.
MEASURE MULTIPLE CYTOKINES
Our Multiplex Cytokine Assay allows simultaneous functional interrogation of T cells as they interact with target cells, giving you insight into the heterogenous mechanisms that regulate cytokine secretion.
Cytokine assays and cell surface markers can be customized.
VISUALIZE TUMOR CELL KILLING
Our Cytotoxicity Assay leverages caspase-3 activation in target cells to visualize the disparate killing behaviors in your T cell samples.
Now, you can develop therapeutics to mediate more effective tumor destruction by the few T cells that matter.
Recover cells of interest alive
After functional characterization, cells of interest can be exported alive for expansion or for further study to link their behavior to genotype.
Connect function to genotype
The TCRseq Well Plate kit allows you to recover your selected cells from fewer than 10,000 cells, amplify TCR alpha and beta chains, and sequence only the T cells that are potential therapeutics.
CONNECT PHENOTYPE TO GENE EXPRESSION
The OptoSeq™ 3′ mRNA kit is the first of its kind — a combined multi-omics workflow which makes it possible to perform cytokine secretion analysis and gene expression profiling on the same cell.
Related Resources
View All Cell Therapy Development ResourcesBrochure: Cell Therapy Development
From expanding rare, antigen-specific T cells to fully characterizing them, learn how our capabilities are making cell therapy development more efficient.
Application Note: Profile and Recover Polyfunctional T Cells Using Multiplexed Cytokine Secretion Assays
Learn how you can easily profile multiple secreted cytokines from live T cells and multiplex with other functional assays.
App Note: Perform Multi-omic Analysis to Directly Link T Cell Polyfunctionality to Gene Expression
Download this application note to learn about designing effective therapeutics by directly linking phenotype to gene expression using the OptoSeq™ Single Cell 3ʹ mRNA kit in the Opto™ Cell Therapy Development workflow.
Get in Touch with Berkeley Lights
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