Workflow
T Cell Profiling
Comprehensive profiling with recovery of cells of interest
customer webinars
Workflow In Action
See how our customers are applying the T cell workflow to develop a deeper understanding of T cell biology.
Featured:
Medical College of Wisconsin on: “Characterizing T Cell Functionality on the Berkeley Lights Lightning Platform”
Watch More:
Children’s Hospital of Philadelphia on How Immune Systems Empower Cancer Immunotherapy
Maximize Functional Characterization
of Your Precious T Cell Samples
The Opto® T Cell Profiling workflow accelerates the creation of better therapeutics by performing multiple functional assays across thousands of individual T cells in a matter of days. Deeply characterize, profile, and map your T cell populations at the single-cell level to directly link phenotype to gene expression.
The importance of a single t cell
One T cell could be the key to the next powerful therapeutic.
To realize its potential, you need to deeply characterize it. The Opto T Cell Profiling workflow lets you directly link single-cell phenotype and function to gene expression. It only takes one cell to create a powerful therapy. We’ll help you find it.
Sort, select and load cells
Allowing desired phenotypes to be selected from heterogeneous T cells samples
Specific cells are targeted based on size, viability and cell surface markers. This gentle sorting process does not require repeated media exchange, which can reduce sorter induced cell stress.
Comprehensive Profiling
Characterize polyfunctionality, cytotoxicity, genotype, and gene expression
The Opto T Cell Profiling workflow makes it possible to comprehensively profile single T cells and link this phenotypic and functional data to the genotype of those same cells.
Download Datasheets
Multiplex Cytokine Assay Datasheet (PDF)
Cytotoxicity Assay Datasheet (PDF)
TCRseq Kit Datasheet (PDF)
MEASURE MULTIPLE CYTOKINES
Our Multiplex Cytokine Assay allows simultaneous functional interrogation of T cells as they interact with target cells, giving you insight into the heterogenous mechanisms that regulate cytokine secretion.
Cytokine assays and cell surface markers can be customized.
VISUALIZE TUMOR CELL KILLING
Our Cytotoxicity Assay leverages caspase-3 activation in target cells to visualize the disparate killing behaviors in your T cell samples.
Now, you can develop therapeutics to mediate more effective tumor destruction by the few T cells that matter.
Recover cells of interest
After functional characterization, cells of interest can be exported for expansion or for further study to link their behavior to genotype.
Link function to genotype
The TCRseq Well Plate kit allows you to recover your selected cells from fewer than 10,000 cells, amplify TCR alpha and beta chains, and sequence only the T cells that are potential therapeutics.
LINK PHENOTYPE TO GENE EXPRESSION
The OptoSeq™ 3′ mRNA kit is the first of its kind — a combined multi-omics workflow which makes it possible to perform cytokine secretion analysis and gene expression profiling on the same cell.
ANTIGEN-SPECIFIC T CELL EXPANSION
The Opto® Antigen Presenting Bead (Opto APB) Kit, HLA*02:01 can be used to measure binding of putative antigenic peptides to HLA Class I, measure stability of peptide-HLA complexes over time, and, in conjunction with costimulatory antibodies pre-conjugated to the APBs, efficiently stimulate and expand endogenous antigen-specific T cells. Antigen-specific T cells are detected using a fluorescently labeled tetramer, which can be purchased directly from MBL International.
Related Resources
View All T Cell Profiling ResourcesBROCHURE: Comprehensive Single T Cell Profiling
Polyfunctionality and cytotoxicity with recovery of live T cells from an integrated platform.
Application Note: Profile and Recover Polyfunctional T Cells Using Multiplexed Cytokine Secretion Assays
Learn how you can easily profile multiple secreted cytokines from live T cells and multiplex with other functional assays.
App Note: Perform Multi-omic Analysis to Directly Link T Cell Polyfunctionality to Gene Expression
Download this application note to learn about designing effective therapeutics by directly linking phenotype to gene expression using the OptoSeq™ Single Cell 3ʹ mRNA kit in the Opto™ Cell Therapy Development workflow.
Get in Touch with Berkeley Lights
Let us know what you are looking for and one of our experts will contact you promptly.