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customer webinars

Workflow In Action

See how our customers are applying the T cell workflow to develop a deeper understanding of T cell biology.


Medical College of Wisconsin on: “Characterizing T Cell Functionality on the Berkeley Lights Lightning Platform”

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Children’s Hospital of Philadelphia on How Immune Systems Empower Cancer Immunotherapy

Maximize Functional Characterization
of Your Precious T Cell Samples

The Opto® T Cell Profiling workflow accelerates the creation of better therapeutics by performing multiple functional assays across thousands of individual T cells in a matter of days. Deeply characterize, profile, and map your T cell populations at the single-cell level to directly link phenotype to gene expression.​

The importance of a single t cell

One T cell could be the key to the next powerful therapeutic.

To realize its potential, you need to deeply characterize it. The Opto T Cell Profiling workflow lets you directly link single-cell phenotype and function to gene expression. It only takes one cell to create a powerful therapy. We’ll help you find it.

  • The Scientist 2019 Top 10 Innovations
    Cruickshank Quinn
    Being able to visualize phenotypes and perform functional assays on thousands of individual cells simultaneously allows users to obtain results in a fraction of the time. Very promising technology for cancer research.
Sort, select and load cells

Allowing desired phenotypes to be selected from heterogeneous T cells samples

Specific cells are targeted based on size, viability and cell surface markers. This gentle sorting process does not require repeated media exchange, which can reduce sorter induced cell stress.

Comprehensive Profiling

Characterize polyfunctionality, cytotoxicity, genotype, and gene expression

The Opto T Cell Profiling workflow makes it possible to comprehensively profile single T cells and link this phenotypic and functional data to the genotype of those same cells.

Download Datasheets

Multiplex Cytokine Assay Datasheet (PDF)
Cytotoxicity Assay Datasheet (PDF)
TCRseq Kit Datasheet (PDF)

Multiplex Cytokines

Our Multiplex Cytokine Assay allows simultaneous functional interrogation of T cells as they interact with target cells, giving you insight into the heterogenous mechanisms that regulate cytokine secretion.

Cytokine assays and cell surface markers can be customized.


Our Cytotoxicity Assay leverages caspase-3 activation in target cells to visualize the disparate killing behaviors in your T cell samples.

Now, you can develop therapeutics to mediate more effective tumor destruction by the few T cells that matter.

Recover cells of interest

After functional characterization, cells of interest can be exported for expansion or for further study to link their behavior to genotype.​

Link function to genotype

The TCRseq Well Plate kit allows you to recover your selected cells from fewer than 10,000 cells, amplify TCR alpha and beta chains, and sequence only the T cells that are potential therapeutics.

Genetic Mechanisms

The OptoSeq™ 3′ mRNA kit is the first of its kind — a combined multi-omics workflow which makes it possible to perform cytokine secretion analysis and gene expression profiling on the same cell.


The Opto® Antigen Presenting Bead (Opto APB) Kit, HLA*02:01 can be used to measure binding of putative antigenic peptides to HLA Class I, measure stability of peptide-HLA complexes over time, and, in conjunction with costimulatory antibodies pre-conjugated to the APBs, efficiently stimulate and expand endogenous antigen-specific T cells. Antigen-specific T cells are detected using a fluorescently labeled tetramer, which can be purchased directly from MBL International.

Antigen-specific T Cell Expansion

Get in Touch with Berkeley Lights

Let us know what you are looking for and one of our experts will contact you promptly.